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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in many tumors, which leads to chromosome instability, and so may well lead to tumorigenesis. This protein was located to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a important job in marketing mobile-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are actually explained. Two additional splice variants were noted but comprehensive nucleotide sequence data will not be nonetheless offered.
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Blocking peptides are peptides that bind particularly for the target antibody and block antibody binding. These peptide generally includes the epitope acknowledged from the antibody.
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This guidebook outlines further probable optimization of mobile and immunoassay parameters to be sure the absolute best final results are...
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This guide outlines further more attainable optimization of mobile and immunoassay parameters to make sure the very best outcomes are...
Antibodies bound to the blocking peptide now not bind on the epitope to the focus on protein. By comparing the staining within the blocked antibody versus the antibody by yourself, you can see which staining get more info is particular.
Goal Information Cyclin E belongs on the highly conserved cyclin family, whose users are characterised by a spectacular periodicity in protein abundance with the mobile cycle. Cyclins purpose as regulators of CDK kinases. Different cyclins exhibit unique expression and degradation designs which add into the temporal coordination of every mitotic event. Cyclin E kinds a complex with and features as being a regulatory subunit of CDK2, whose action is required for cell cycle G1/S changeover. This protein accumulates at the G1-S stage boundary which is degraded as cells progress by way of S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by each antibodies...
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